LABORATORY OF PHYSIOLOGICAL ANATOMY
| team leader: |
RNDr. Aleš Soukup, PhD. |
| members: |
RNDr. Edita Tylová, RNDr. Olga Votrubová, Csc. |
| PhD. students : |
RNDr. Eva Pecková |
| MSc. students: |
Bc. Tereza Šnajdrová, Bc. Daniela Halamková |
| technician: |
Ing. Yvetta Šefrnová |
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Our work is ground on the “traditional” plant anatomy and its methodological approaches, which was gradually enriched for methods of cytology, biochemical analysis and molecular biology. Such a combination allows for connection of structural parameters of plant body with its functions. Plant body structure is not rigid but undergoes changes related to tissue differentiation, plant ontogeny or factors of environment. Observed responses are therefore studied and interpreted from environmental and developmental point of view and associated mechanisms of regulations. Our interest is concentrated on the study of “hidden half” - root system, its development, function and interaction with the rhizosphere. |
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used methods:
- cultivation of model plants (mainly Arabidopsis, maize, rice, Phragmites, Glyceria, Acorus etc.) in hydroponic cultures, soil or in vitro; cultivation of root cultures in vitro
- methods of classical plant anatomy (permanent (paraffin, resin) and vital slides, histochemical methods, etc.)
- microscopic techniques (light microscopy incl. florescent and confocal microscopy, electron microscopy), image processing and quantitative image analysis
- cytological techniques (specific florescent probes, imunolocalisation etc.)
- selected methods of molecular biology, use of transgenic plants
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| Defended |
| 2012 | Tereza Šnajdrová: Lateral roots and root nodules – similarities and differences |
| 2011 | Daniela Halamková: Regulation of lateral root initiation depending on the availability of crucial mineral nutrients in the environment |
| 2009 | Alois Antonín Hilgert Delgado : Regulation of lateral root development |
| 2007 | Lenka Vinšová (Moravcová): Changes of apical meristem within the growing root |
| 2006 | Jitka Čierná (Padruňková): Effect of flooding on growth and plant structure |
| 2006 | Eva Pecková (Husáková): Lateral roots and root nodules – similarities and differences |
| Offered |
Plant membranes polarity and its importance in communication with the environment
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Stress-induced morphogenic changes of root system
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Regulation of functional root and shoot ratio
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Apoplastic barriers in roots versus uptake of nutrients
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| In progress |
| Daniela Halamková: Signaling mechanisms of regulation of lateral root development in response to nutrient availability in the environment. |
| Tereza Šnajdrová: Characterisation of candidate genes of line MGT180 and their participation in the formation of lateral roots |
| Defended |
| 2012 | Barbora Ježková (Gorčicová): Comparative anatomy of Lasiocephalus from paramos, Equador |
| 2011 | Alois Antonín Hilgert Delgado: Function of TTL gene family and their effect on lateral root formation of Arabidopsis |
| 2008 | Kateřina Machačová: Arrangement of microtubular cytoskeleton during development of Casparian band in endodermal cells of root of Allium cepa |
| 2008 | Eva Pecková (Husáková): Structural changes of maize root system; effect of phytohormones |
| 2007 | Kateřina Vášková: Formation of symplastic domains in primary cortex of the developing root |
| Offered |
| Study of initiation and growth of Arabidopsis thaliana lateral roots and characterisation of genes with corresponding expression pattern |
| In progress |
| Eva Pecková (Husáková): Structural and functional characterisation of the maize lrt1 mutant |
| Defended |
| 2012 | Zuzana Lenochová: Characterization of programmed cell death during lyzigenous aerenchyma formation |
| 2011 | Edita Tylová (Munzarová): Nitrogen acquisition in wetland plants (nutritional aspects of plant performance under eutrophication) |
| 2006 | Lenka Steinbachová (Vojtíšková): Carbohydrate metabolism of selected wetland plants-relation to their growth and mineral nutrition |
Students interested in the field are very welcome. If you are interested in particular topic not listed above, feel free contact us.
We will be pleased if you contact us : asoukup@natur.cuni.cz
| National cooperation |
Masaryk University in Brno (Doc. Kummerová, Dr. Baláž, Dr. Gloser)
University of South Bohemia in České Budějovice (Doc. Šantrůček, Dr. Čížková)
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| International cooperation |
Comenius University in Bratislava (Prof. Lux)
State University of New York (prof. Seago)
Instituto de Biotecnologia, UNAM, Cuernavaca, Mexiko (Dr. Dubrovsky)
University of Hull, UK (prof. Armstrong)
University of Bonn, Germany (prof. Schreiber, prof. Hochholdinger) |
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