LABORATORY OF ECOPHYSIOLOGICAL ANATOMY
Head: Dr. Jana Albrechtová, Associate Professor
Team members: Dr. Zuzana Lhotáková, MSc. Petr Kohout
Ph.D. student: MSc. Zuzana Kubínová
MSc. students: Monika Kovářová, Marie Otradovcová, Tereza Lukešová, Tomáš Antl, Jana Horská, Anna Vrbová, Libor Sedlecký, Zuzana Mahrlová, Markéta Pikorová
Technicians: MSc. Drahomíra Bartáková


 Our research focuses on study of plant response to impact of environmental biotic and biotic factors, considering the changes in plant physiological processes (photosynthesis, accumulation of secondary metabolites) and changes in plant structure (primarily leaf anatomy).

 We concentrate our research mainly on tree ecophysiology. For study of structural changes we use methods of quantitative anatomy and contribute to development or adjustment of these methods. We use wide spectrum of methodical approaches enabling to conduct our research at several hierarchical levels – starting with microcopic, histochemical and biochemical techniques, to optical leaf properties, which can be applied in remote sensing. Our research is focused in two main topics:

1) Study of leaf physiology, structure and leaf optical properties under impadt of environmental factors – elevated CO2 concentration, irradiance and soil trace element contamination.

Current projects:
  • Assessment of Mining Related Impacts Based on Utilization of HyMap Airborne Hyperspectral Sensor. GA CR 205/09/1989 (2009-2012)
  • Impact of CO2 enrichment and irradiance on structure and performance of forest tree photosynthetic apparatus at different hierarchical levels. GA CR P501/10/0340 (2010-2014)

2) Study of ectomycorrhizal symbioses and associations. We fosuce on the utilisation of mycorrhizal inoculations in agriculture production and on role of mycorrizal symbioses in coniferour forests.

Current topics and projects:

Application of mycorrhizal inoculations in agriculture production; in colaboration with Symbiom company and Dr. Vosátka from the Institute of Botany, Academy of Sciences of CR.
  • COST OC09057 Development of sustainable production scheme of selected vegetable species on the basis of mycorrhizal biotechnology
  • COST Action 870 “From production to application of arbuscular mycorrhizal fungi in agricultural systems: a multidisciplinary approach” http://www.cost.esf.org/domains_actions/fa/Actions/870

The role of mycorrhizal symbioses and associations in coniferous forest, focuse on invasive tree species, in collaboration with the Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of Czech Republic
  • COST OC10058 Roots, ectomycorrhizas, and below ground carbon balance of Norway spruce forests of Central Europe COST action FP0803 http://www.wsl.ch/fe/boden/projekte/cost-action/organization_EN
  • GAUK 2011/320311 Role of DSE (Dark Septate Endophytes) in plant community of forest ecosystem


 We use following methodical approaches:
- biochemical methods (spectrophotometrical detection of photosynthetic pigments, phenolic compounds, lignin)
- light microscopy, histochemical detections
- confocal microscopy
- transmission electron microscopy
- stereological methods (estimation of mesophyll geometrical parameters – e.g. inner leaf surface, chloroplast number), image analysis
- fungal DNA isolation, amplification and sequencing
- isolation of fungal mycobionts from host plants

Bachelor theses
PhD theses
In progress Zuzana Kubínová: Studium of changes of structural charcteristics of photosynthetic appatatus of selected wood species under the effect of elevated CO2 and different irradiance
Defended Zuzana Lhotáková (2009): Study of Coniferous Needles in Relation to Environmental Factors Using Approaches of Quantitative Anatomy and Laboratory Spectroscopy
  Martin Vohník (2007): Interactions in the rhizosphere of ericoid mycorrhizal plants
  Sándor Tamás Forczek (2006): Secondary Atmospheric Pollutants and Forest Decline: Uptake, Effect and Fate of Trichloroacetic Acid in Norway Spruce (Picea abies/L./Karst)
  Enkhtuya Batkhuu (2005): Influence of arbuscular mycorrhizal symbiosis on ecophysiology of trees and grass in disturbed ecosystems
  Tomáš Polák (2005): Study of Macroscopic Markers of Norway Spruce Damage and Recovery

Collaboration on projects and student supervising at the department level: Collaboration with team of Dr. Helena Lipavská (Laboratory of Plant Morphogenesis Regulatory Factors) – joint project, student supervising)

Collaboration with Dr. Martin Vohník (selected mycorrhizal topics, joint projects, student supervising)

Collaboration with Dr. Věra Čížková (student supervising – diploma theses for students of Education and Didactics of Biology)
Collaboration at the faculty level: Department of Genetics and Microbiology – Dr. Holá, Dr. Kočová (joint project)

Department of Applied Geoinformatics and Cartography – Dr. Potůčková, Dr. Kupková (joint project)

Institute for Environmental Studies – Dr. Frouz (joint project since 2012)
Domestic collaboration: Institute of Botany, AS CR, v.v.i. : Dr. M. Vosátka, Mgr. Kohout, Dr. Vohník (several joint projects)

Institute of Physiology, AS CR, v.v.i.: Dr. L. Kubínová, Dr. J. Janáček, Dr. Radochová

Global Change Research Centre AS CR, v.v.i.: , Ing. Hanuš, Dr. Šprtová, Dr. Urban (joint project), doc. P. Cudlín (former joint projects, COST), prof. M.V. Marek

Czech Geological Survey - Mgr. V Kopačková, Mgr. J. Mišurec (joint project)

Institute of Microbiology AS CR, v.v.i.: Dr. Baldrián (joint project since 2012)

University of South Bohemia, Faculty of Science, Department of Plant Physiology and Anatomy, (doc. J. Šantrůček)
Foreign collaboration Martin- Luther University, Halle, Germany – prof. C. Glaesser

University of Zurich, Switzerland– Dr. Z. Malenovský

University of New Hampshire, USA - BN. Rock, S. Ollinger

University of Maryland, NASA Goddard Space Flight Center, USA – P.Entcheva-Campbell

University of Tartu (Estonia) – Dr. Tedersoo

Szent István University (Hungary) – Dr. K. Posta

University of Lund (Sweden) – Dr. Wallander