Head: RNDr. Helena Lipavská, PhD.
Team members: RNDr. Hana Konrádová, PhD; RNDr. Petra Mašková; Mgr. Petra Vojvodová
MSc students: Klára Čiháková, Daniel Krsek, Ondřej Skala
Technician: Olga Činglová

 At the first place, the research group deals with plant sugar metabolism in context with developmental changes and stress conditions (changes in saccharide metabolism during conifer somatic embryogenesis, involvement of sugar alcohols and raffinose family oligosaccharide pathway (RFO) in plant stress response, investigation on the signal role of saccharides in plant morphogenesis). The group also focuses on developmental changes of transgene plant material with an implemented foreign mitotic activator (the influence of transformation on growth characteristics and organogenesis de novo in tobacco and Arabidopsis cultures, cell characteristics of suspension cultures, flowering regulation in photoperiodically neutral tobacco.

 Model organisms:
- cell and tissue cultures (tobacco, Arabidopsis, apple tree, celery)
- embryogennic cultures (spruce, fir)
- in vitro cultivated plants (potato, olive, celery, tobacco)

 Methodical approaches:
- cultivation of whole plants, organic, tissue and cell cultures in in vitro conditions
- grafting technology
- basic anatomical and cytological analysis (image analysis)
- liquid chromatography (HPLC) (analysisof starch content and soluble saccharides spectra)
- histochemical localization of starch and lipid compounds
- biochemical determination of the activity of the saccharide metabolism key enzymes
- histochemical localization of the activity of the saccharide metabolism key enzymes

Bachelor theses:
offered Somatic Embryogenesis in Dicotyledon Plants – the Role of Signalling Compounds, Saccharides and Phytohormones

The Potato Tuberization Regulating Factors

Pollen Embryogenesis

The Regulation of Cell Cycle: The Role of the Compounds with Signaling Nature
recently defended (2006/2007) Jan Ponert: The Influence of Cytokinins on saccharide Metabolism and the Interaction of Saccharide and Cytokinin Signaling

Hana Ševčíková: Mechanisms of Saccharide Sensing and Saccharide Signaling in Plants
Diploma theses
in progress Klára Čiháková: The Influence of the Transformation with cdc25 Gene on the Plant Root System Structure

Daniel Krsek: The Role of Saccharides in Abiotic Stress in Plants with a Huge Diversity in Their Photosynthetic Products

Ondřej Skala: Somatic Embryogenesis of Coniferous Trees: the Role of Raffinose Family Oligosaccharide Pathway in the Process Progress
recently defended Lenka Šuťáková: The Reaction on Abiotic Stress in Plants with a Huge Diversity in Their Photosynthetic Products.

Lucie Uchytilová: The Influence of the Transformation with cdc25 Gene from Schizosaccharomyces pombe on the Morphogenesis of Nicotiana tabacum L., cv. Samsun

Nikola Drážná: Somatic Embryogenesis of Norway Spruce (Picea abies (L.) Karst.): the Impact of Changes in Saccharide Availability during the Maturation on Liquid Media on the Yield and Quality of the Embryos
PhD theses
in progress Petra Vojvodová: The Regulation of Neutral Plants Flowering: Analysis of Physiological Factors and Flowering Regulation Genes that Are Involved in Spcdc25 Transgenic Tobacco Plants.
recently defended Edita Tylová (roz. Munzarová): Nitrogen acquisition in wetland plants (nutritional aspects of plant performance under eutrophication)

Domestic collaboration Institute of Experimental Botany ASCR, the Ass. Prof. Strnad group
Foreign collaboration Institute of Plant Genetics and Biotechnology, SAS, Nitra, Dr. Salajová;
Department of Plant Physiology, University of Wageningen, the Netherlands (Dr. Vreugdenhil);
School of Biosciences, Cardiff University, Great Britain (Dr. Francise);